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KMID : 0043320160390040547
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Archives of Pharmacal Research
2016 Volume.39 No. 4 p.547 ~ p.554
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Yin-Yang 1 and Yin-Yang 2 exert opposing effects on the promoter activity of interleukin 4
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Lee Sung-Ho
Cho Young-Chang
Jeong Hyung-Min
Kim Kyung-Hee
Choi Hyun-Jin
Lee Kwang-Youl
Kang Bok-Yun
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Abstract
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Interleukin (IL)-4 acts on T cells as a growth and activation factor, and promotes the differentiation of type 2 T helper cells. In T cells, expression of the gene encoding IL-4 is regulated by inducible or constitutive factors. Yin-Yang (YY)-1 is one of constitutive transcription factors binding to the IL-4 promoter. The recently identified YY2 protein is similar to YY1, with both sharing high levels of homology in their zinc finger motifs. However, the role of YY2 in T cells is unclear. YY1 and YY2 were constitutively expressed in EL4 T cells, and their expression was not dependent on stimulation. IL-4 promoter (?741/+56 fragment) activity was enhanced by YY1, but inhibited by YY2. The enhanced IL-4 promoter activity by YY1 was reduced by simultaneous expression of YY2. In addition, the DNA binding affinity of YY1 to the IL-4 promoter was adversely affected by YY2. Our results suggest that YY1 and YY2 exert opposing effects on the IL-4 promoter as they compete for the same DNA binding sites.
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KEYWORD
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YY1, YY2, IL-4, Th2 cell, Constitutive factor
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